RNA

Part:BBa_J64722:Design

Designed by: Sheel Dandekar   Group: Voigt Lab   (2007-03-20)

taR12 key


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 85
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 85
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was modified from Isaacs et. al., "Engineered riboregulators enable post-transcriptional control of gene expression." Nature Biotechnology, 2004. The 5' end of the sequence was then extended and changed to basepair with the extended 5' end of J64720. Additionally, the 3' end was extended and designed to basepair with the 3' extension of J64720. Lastly, the hairpin in this constuct will basepair with the hairpin found in J64720.


Source

Isaacs et. al., "Engineered riboregulators enable post-transcriptional control of gene expression." Nature Biotechnology, 2004.


References